| GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases SQ Tsai, Z Zheng, NT Nguyen, M Liebers, VV Topkar, V Thapar, ... Nature Biotechnology, 2014 | 2246 | 2014 |
| Engineered CRISPR-Cas9 nucleases with altered PAM specificities BP Kleinstiver, MS Prew, SQ Tsai, VV Topkar, NT Nguyen, Z Zheng, ... Nature 523 (7561), 481-485, 2015 | 1861 | 2015 |
| CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets SQ Tsai, NT Nguyen, J Malagon-Lopez, VV Topkar, MJ Aryee, JK Joung Nature methods 14 (6), 607-614, 2017 | 716 | 2017 |
| Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition BP Kleinstiver, MS Prew, SQ Tsai, NT Nguyen, VV Topkar, Z Zheng, ... Nature biotechnology 33 (12), 1293-1298, 2015 | 701 | 2015 |
| Dimeric CRISPR RNA-guided FokI-dCas9 nucleases directed by truncated gRNAs for highly specific genome editing N Wyvekens, VV Topkar, C Khayter, JK Joung, SQ Tsai Human gene therapy 26 (7), 425-431, 2015 | 193 | 2015 |
| Accelerated cryo-EM-guided determination of three-dimensional RNA-only structures K Kappel, K Zhang, Z Su, AM Watkins, W Kladwang, S Li, G Pintilie, ... Nature methods 17 (7), 699-707, 2020 | 136 | 2020 |
| Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics K Leppek, GW Byeon, W Kladwang, HK Wayment-Steele, CH Kerr, AF Xu, ... Nature communications 13 (1), 1536, 2022 | 125 | 2022 |
| Replacing uridine with 2-thiouridine enhances the rate and fidelity of nonenzymatic RNA primer extension BD Heuberger, A Pal, F Del Frate, VV Topkar, JW Szostak Journal of the American Chemical Society 137 (7), 2769-2775, 2015 | 76 | 2015 |
| Open-source guideseq software for analysis of GUIDE-seq data SQ Tsai, VV Topkar, JK Joung, MJ Aryee Nature Biotechnology 34 (5), 483-483, 2016 | 74 | 2016 |
| Defining genome-wide CRISPR–Cas genome-editing nuclease activity with GUIDE-seq NL Malinin, GH Lee, CR Lazzarotto, Y Li, Z Zheng, NT Nguyen, M Liebers, ... Nature protocols 16 (12), 5592-5615, 2021 | 34 | 2021 |
| Transcription factors, coregulators, and epigenetic marks are linearly correlated and highly redundant T Ahsendorf, FJ Müller, V Topkar, J Gunawardena, R Eils PLoS One 12 (12), e0186324, 2017 | 17 | 2017 |
| mRNA transport and local translation in glia LM Meservey, VV Topkar, M Fu Trends in cell biology 31 (6), 419-423, 2021 | 14 | 2021 |
| Three-dimensional structure-guided evolution of a ribosome with tethered subunits DS Kim, A Watkins, E Bidstrup, J Lee, V Topkar, C Kofman, KJ Schwarz, ... Nature chemical biology 18 (9), 990-998, 2022 | 11 | 2022 |
| Anomalous reverse transcription through chemical modifications in polyadenosine stretches W Kladwang, VV Topkar, B Liu, R Rangan, TL Hodges, SC Keane, ... Biochemistry 59 (23), 2154-2170, 2020 | 10 | 2020 |
| Structural Determinants of MRNA Transport Specificity in Oligodendrocytes VV Topkar Biophysical Journal 118 (3), 67a, 2020 | | 2020 |
Shengdar Q. TsaiAssociate Member, St. Jude Children's Research HospitalVerified email at stjude.org
