| Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA F Sousa, DMF Prazeres, JA Queiroz Trends in biotechnology 26 (9), 518-525, 2008 | 181 | 2008 |
| Minicircle DNA vectors for gene therapy: advances and applications V Gaspar, D Melo-Diogo, E Costa, A Moreira, J Queiroz, C Pichon, ... Expert opinion on biological therapy 15 (3), 353-379, 2015 | 109 | 2015 |
| Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography F Sousa, DMF Prazeres, JA Queiroz The journal of gene medicine 11 (1), 79-88, 2009 | 108 | 2009 |
| Selective purification of supercoiled plasmid DNA from clarified cell lysates with a single histidine–agarose chromatography step F Sousa, S Freitas, AR Azzoni, DMF Prazeres, J Queiroz Biotechnology and applied biochemistry 45 (3), 131-140, 2006 | 108 | 2006 |
| Bioreducible poly (2-ethyl-2-oxazoline)–PLA–PEI-SS triblock copolymer micelles for co-delivery of DNA minicircles and Doxorubicin VM Gaspar, P Baril, EC Costa, D de Melo-Diogo, F Foucher, JA Queiroz, ... Journal of Controlled Release 213, 175-191, 2015 | 101 | 2015 |
| Pichia pastoris: a recombinant microfactory for antibodies and human membrane proteins AM Gonçalves, AQ Pedro, C Maia, F Sousa, JA Queiroz, LA Passarinha J Microbiol Biotechnol 23 (5), 587-601, 2013 | 97 | 2013 |
| Advances in chromatographic supports for pharmaceutical‐grade plasmid DNA purification  Sousa, F Sousa, JA Queiroz Journal of separation science 35 (22), 3046-3058, 2012 | 95 | 2012 |
| Formulation of chitosan–TPP–pDNA nanocapsules for gene therapy applications VM Gaspar, F Sousa, JA Queiroz, IJ Correia Nanotechnology 22 (1), 015101, 2010 | 93 | 2010 |
| Recombinant pre-miR-29b for Alzheimer s disease therapeutics PA Pereira, JF Tomás, JA Queiroz, AR Figueiras, F Sousa Scientific reports 6, 19946, 2016 | 91 | 2016 |
| Poly (2-ethyl-2-oxazoline)–PLA-g–PEI amphiphilic triblock micelles for co-delivery of minicircle DNA and chemotherapeutics VM Gaspar, C Gonçalves, D de Melo-Diogo, EC Costa, JA Queiroz, ... Journal of Controlled Release 189, 90-104, 2014 | 89 | 2014 |
| Cervical cancer and HPV infection: ongoing therapeutic research to counteract the action of E6 and E7 oncoproteins AM Almeida, JA Queiroz, F Sousa, Â Sousa Drug Discovery Today 24 (10), 2044-2057, 2019 | 86 | 2019 |
| Ribonucleic acid purification R Martins, JA Queiroz, F Sousa Journal of Chromatography A 1355, 1-14, 2014 | 85 | 2014 |
| Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography F Sousa, T Matos, DMF Prazeres, JA Queiroz Analytical biochemistry 374 (2), 432-434, 2008 | 85 | 2008 |
| Nanoparticle mediated delivery of pure P53 supercoiled plasmid DNA for gene therapy VM Gaspar, IJ Correia, Â Sousa, F Silva, CM Paquete, JA Queiroz, ... Journal of controlled release 156 (2), 212-222, 2011 | 83 | 2011 |
| Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine-agarose support F Sousa, CT Tomaz, DMF Prazeres, JA Queiroz Analytical biochemistry 343 (1), 183-185, 2005 | 80 | 2005 |
| Folate-targeted multifunctional amino acid-chitosan nanoparticles for improved cancer therapy VM Gaspar, EC Costa, JA Queiroz, C Pichon, F Sousa, IJ Correia Pharmaceutical research 32 (2), 562-577, 2015 | 74 | 2015 |
| Differential interactions of plasmid DNA, RNA and genomic DNA with amino acid‐based affinity matrices A Sousa, F Sousa, JA Queiroz Journal of separation science 33 (17‐18), 2610-2618, 2010 | 67 | 2010 |
| Influence of growth conditions on plasmid DNA production F Silva, L Passarinha, F Sousa, JA Queiroz, FC Domingues J Microbiol Biotechnol 19 (11), 1408-1414, 2009 | 66 | 2009 |
| Successful application of monolithic innovative technology using a carbonyldiimidazole disk to purify supercoiled plasmid DNA suitable for pharmaceutical applications A Sousa, CT Tomaz, F Sousa, JA Queiroz Journal of Chromatography A 1218 (46), 8333-8343, 2011 | 57 | 2011 |
| Amino acids–nucleotides biomolecular recognition: from biological occurrence to affinity chromatography F Sousa, C Cruz, JA Queiroz Journal of Molecular Recognition 23 (6), 505-518, 2010 | 57 | 2010 |
