Ben Kleinstiver
Ben Kleinstiver
Assistant Professor, Massachusetts General Hospital; Assistant Professor, Harvard Medical School
Verified email at mgh.harvard.edu - Homepage
TitleCited byYear
High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
BP Kleinstiver, V Pattanayak, MS Prew, SQ Tsai, NT Nguyen, Z Zheng, ...
Nature 529 (7587), 490-495, 2016
11632016
Engineered CRISPR-Cas9 nucleases with altered PAM specificities
BP Kleinstiver, MS Prew, SQ Tsai, VV Topkar, NT Nguyen, Z Zheng, ...
Nature 523 (7561), 481-485, 2015
8142015
Enhanced proofreading governs CRISPR–Cas9 targeting accuracy
JS Chen, YS Dagdas, BP Kleinstiver, MM Welch, AA Sousa, ...
Nature 550 (7676), 407, 2017
2932017
Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition
BP Kleinstiver, MS Prew, SQ Tsai, NT Nguyen, VV Topkar, Z Zheng, ...
Nature Biotechnology, 2015
2842015
Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells
BP Kleinstiver, SQ Tsai, MS Prew, NT Nguyen, MM Welch, JM Lopez, ...
Nature Biotechnology 34 (8), 869-874, 2016
2702016
Inducible and multiplex gene regulation using CRISPR–Cpf1-based transcription factors
YE Tak, BP Kleinstiver, JK Nuñez, JY Hsu, JE Horng, J Gong, ...
nature methods 14 (12), 1163, 2017
572017
Isocitrate dehydrogenase mutations confer dasatinib hypersensitivity and SRC dependence in intrahepatic cholangiocarcinoma
SK Saha, JD Gordan, BP Kleinstiver, P Vu, MS Najem, JC Yeo, L Shi, ...
Cancer discovery 6 (7), 727-739, 2016
542016
Monomeric site-specific nucleases for genome editing
BP Kleinstiver, JM Wolfs, T Kolaczyk, AK Roberts, SX Hu, DR Edgell
Proceedings of the National Academy of Sciences 109 (21), 8061-8066, 2012
532012
Discovery of widespread type I and type V CRISPR-Cas inhibitors
ND Marino, JY Zhang, AL Borges, AA Sousa, LM Leon, BJ Rauch, ...
Science 362 (6411), 240-242, 2018
462018
Hypoxia drives transient site-specific copy gain and drug-resistant gene expression
JC Black, E Atabakhsh, J Kim, KM Biette, C Van Rechem, B Ladd, ...
Genes & development 29 (10), 1018-1031, 2015
452015
Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs
J Listgarten, M Weinstein, BP Kleinstiver, AA Sousa, JK Joung, J Crawford, ...
Nature Biomedical Engineering 2 (1), 38, 2018
392018
Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
K Lee, Y Zhang, BP Kleinstiver, JA Guo, MJ Aryee, J Miller, A Malzahn, ...
Plant biotechnology journal 17 (2), 362-372, 2019
302019
Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
BP Kleinstiver, AA Sousa, RT Walton, YE Tak, JY Hsu, K Clement, ...
Nature biotechnology 37 (3), 276, 2019
282019
CRISPR/Cas9 Mediated Disruption of the SwedishAPP Allele as a Therapeutic Approach for Early-Onset Alzheimer’s Disease
B György, C Lööv, MP Zaborowski, S Takeda, BP Kleinstiver, C Commins, ...
Molecular Therapy-Nucleic Acids 11, 429-440, 2018
252018
A unified genetic, computational and experimental framework identifies functionally relevant residues of the homing endonuclease I-BmoI
BP Kleinstiver, AD Fernandes, GB Gloor, DR Edgell
Nucleic acids research 38 (7), 2411-2427, 2010
202010
The I-TevI nuclease and linker domains contribute to the specificity of monomeric TALENs
BP Kleinstiver, L Wang, JM Wolfs, T Kolaczyk, B McDowell, X Wang, ...
G3: Genes, Genomes, Genetics 4 (6), 1155-1165, 2014
192014
Allele-Specific CRISPR-Cas9 Genome Editing of the Single-Base P23H Mutation for Rhodopsin-Associated Dominant Retinitis Pigmentosa
P Li, BP Kleinstiver, MY Leon, MS Prew, D Navarro-Gomez, ...
The CRISPR Journal 1 (1), 55-64, 2018
182018
Strand-specific contacts and divalent metal ion regulate double-strand break formation by the GIY-YIG homing endonuclease I-BmoI
JM Carter, NC Friedrich, B Kleinstiver, DR Edgell
Journal of molecular biology 374 (2), 306-321, 2007
182007
The monomeric GIY-YIG homing endonuclease I-BmoI uses a molecular anchor and a flexible tether to sequentially nick DNA
BP Kleinstiver, JM Wolfs, DR Edgell
Nucleic acids research 41 (10), 5413-5427, 2013
142013
Engineered CRISPR-Cas9 nucleases
JK Joung, B Kleinstiver, V Pattanayak
US Patent 9,512,446, 2016
132016
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